LakePharma Antibody Analyzer

Details on the development and use of this method are described here:

Sean H Gao, Kexin Huang, Hua Tu, and Adam S Adler. (2013) Monoclonal antibody humanness score and its applications. BMC Biotechnology, 13:55.

Chain type



Framework + CDR
Framework only




The T20 score analyzer is a tool that calculates the humanness of monoclonal antibody variable region sequences. The analyzer possesses the ability to consistently distinguish human antibody variable region sequences from other species' antibody sequences, such as murine sequences. The T20 score is scaled from 0 to 100, where a higher score is a more human-like antibody. In general, full-length sequences that score above 80 are considered human-like, while framework only sequences that score above 85 are considered human-like.

The CDR and framework region boundaries are defined according to the Kabat numbering scheme. The antibody sequences that are outputted using the BLAST results or FASTA sequences options are distinguished by their GI number, which can be directly inputted into the NCBI Protein database for identification. It is located at

If there are any issues or questions, please let us know at